FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527916
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT84888415.356311492432228Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT87900.15901109930035118Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA82390.14904350934420857No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79760.1442858393651423Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC76100.13766489939427443RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC72030.13030226942667RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA69420.12558077944744456No Hit
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT67220.1216009794649557Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT59570.10776212952584663No Hit
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT58360.10557323953547776Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1052350.0139.904191
CTGCAAT1051150.0139.21292
CCGCAAT150400.0133.543952
ACCGCAA152400.0132.301641
TGCAATT1214100.0130.475943
CGCAATT164600.0127.978563
GCAATTT1677700.0107.951664
CAATTTC1691200.0107.6096345
AATTTCC1696600.0107.6029366
TTTCCAC1687650.0106.678758
ATTTCCA1704050.0106.21367
TTCCACA1714800.0105.256059
CGGCAAT23600.0100.957542
ACGGCAA27500.086.661921
GGCAATT28000.086.388373
ATTGCAA58700.081.32321
TATGCAA30050.062.140651
ATGCAAT120700.060.904612
GCAATTC40800.054.123084
AATGCAA79650.051.7230871
Produced by FastQC (version 0.11.5)