FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6591805
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT207449231.470773179728468Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT610180.9256645182920308Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT436210.6617459102628187Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT354630.5379861813266624Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT316810.48061191130502195Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT161450.24492532773648493Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC128840.1954548109356997RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC128810.1954092998806852RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103340.15677041417335616Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC101720.15431281720257198Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCCATCTCGTATGCCGTCTT94260.14299573485562755Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC88210.13381767209436565Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC68510.10393207930149632RNA PCR Primer, Index 16 (100% over 22bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT66830.10138346022068309Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT451050.0127.526622
ACTGCAA464750.0123.826451
TGCAATT515000.0123.2618263
CGCAATT68800.0109.8017653
GCAATTT675750.0106.748184
GACCGGA73200.0106.272762
CCGCAAT66950.0105.689682
AATTTCC735850.0105.493596
AGACCGG74900.0104.980321
CAATTTC716400.0104.838565
GATTGGA105700.0104.736342
AGATTGG108050.0102.634231
TTTCCAC755300.0102.130048
ATTTCCA760250.0101.801117
TTCCACA790650.097.535559
ACCGGAA80250.097.11663
ATTGGAA119300.091.884323
CCGGAAG85950.091.012084
TTGGAAG122650.088.9004064
ACCGCAA80900.087.403091
Produced by FastQC (version 0.11.5)