FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14006534
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT251054417.92409171319614Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT597070.42627962063990993Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT278770.19902853910896157Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT240500.1717055768400662Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC150950.10777113024535549RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC149460.10670734101669978RNA PCR Primer, Index 16 (96% over 25bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT144580.1032232528047267Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT897400.0127.966172
TGCAATT985150.0124.695563
ACTGCAA943400.0122.331621
CGCAATT120200.0112.429733
GCAATTT1283950.0107.1737064
AATTTCC1345200.0105.148826
CAATTTC1329800.0104.257715
CCGCAAT124350.0104.248862
TTTCCAC1370850.0102.555078
ATTTCCA1407450.0100.161057
TTCCACA1427000.098.68229
ACCGCAA143850.090.051581
GATTGGA135000.075.467222
AGATTGG137900.074.116431
ATTGGAA142300.071.646673
AGACCGG68050.063.132481
GACCGGA67500.063.014592
TTGGAAG168900.060.1062934
AAGAGCA7840600.053.720088
AGAGCAC7814900.053.6677259
Produced by FastQC (version 0.11.5)