FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005479704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6085625
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTG175050.28764506521515865No Hit
ATGGTT151820.24947314367875115No Hit
GGGGGG148710.24436274006367464No Hit
CTGGGG140510.23088836397247614No Hit
GGTGGG136210.22382253260757934No Hit
TGGTTG130150.21386464003286432No Hit
GGTGCC122720.2016555407209613No Hit
ATGGCT117800.19357091506624216No Hit
TGTTGG114740.18854267228099003No Hit
TGTATA113010.18569990756906646No Hit
GGGGCC108090.17761528191434733No Hit
ATGGTA106460.1749368388620725No Hit
TTGTCA106380.1748053815343535No Hit
AGAGGT105270.17298141111225224No Hit
TGGCGG104200.17122316935401047No Hit
GCGGGT102910.16910341994454142No Hit
GCGGGG99700.16382869466981614No Hit
GTACAT99230.16305638286946697No Hit
ATTGGA97570.16032864331929753No Hit
GCTCGT97510.16023005032350826No Hit
TTTGGG97500.16021361815754337No Hit
TTCTTG96780.1590305022080723No Hit
CGTGCA96550.15865256239088016No Hit
GTGCTG96530.1586196980589504No Hit
GGGAGC96120.15794597925439047No Hit
GCGGAA94130.1546759782273801No Hit
CTGGGA93750.1540515559207148No Hit
TTCGTT92680.15229331416247305No Hit
TGGGGG92600.15216185683475403No Hit
AGAGGG92230.1515538666940536No Hit
AATGCG91920.15104446954914247No Hit
GTGGGG91030.14958200677826847No Hit
TGAGGG90720.1490726096333573No Hit
GGACGT90300.1483824586628325No Hit
GGGGAA90080.14802095101160523No Hit
CTTTGT89750.1474786895347643No Hit
CCCAGG89640.14729793570915067No Hit
TTCGCT89120.1464434630789771No Hit
TATGGT88940.14614768409160933No Hit
GCGTGG88420.14529321146143576No Hit
GGGTTA86610.14231898942179316No Hit
GTAGGT85530.14054431549758653No Hit
GGGGTG85110.13985416452706173No Hit
GGTTGT84670.13913114922460718No Hit
GTTAAC84580.1389832597309233No Hit
TCTGTA84500.13885180240320427No Hit
GGTGGT84270.13847386258601213No Hit
GTGTCT83440.1371099928109274No Hit
GGACAC83340.13694567115127862No Hit
AGGGGA82450.13548320838040465No Hit
TACGGG81870.1345301427544418No Hit
GTGCGC81690.13423436376707404No Hit
CGATGC81240.13349491629865462No Hit
CGGTGT81120.1332977303070761No Hit
AGGGGG80450.1321967751874294No Hit
CGCGCT80160.131720242374448No Hit
GGAGGT79750.13104652356988805No Hit
TAGGCC79680.13093149840813392No Hit
GTGGTG79250.13022491527164426No Hit
GGAGGG78090.1283187840197186No Hit
TTCTGT77940.12807230153024546No Hit
CGGGTC77930.12805586936428057No Hit
GGTTCG77870.1279572763684913No Hit
GGAAGG77860.12794084420252644No Hit
TGTGTT77090.12667556742323097No Hit
GGATGA76880.12633049193796858No Hit
GAAAGG76400.12554174797165452No Hit
CTGGGC76340.12544315497586525No Hit
AGGCTT75720.12442436068604293No Hit
AGTGGG75180.1235370237239396No Hit
AAGGGG74970.12319194823867721No Hit
CGGGGC74630.1226332545958714No Hit
GGGAGG74450.12233747560850365No Hit
ACGGCA74390.1222388826127144No Hit
AGTCCG73680.12107219882920818No Hit
GTAGTC73410.12062853034815653No Hit
GTGAGG73400.12061209818219165No Hit
GGGCCG72980.11992194721166682No Hit
CGCGCC72950.1198726507137722No Hit
TTCGGT72700.11946184656465031No Hit
GGAGTC72500.11913320324535277No Hit
ATGGTC72400.11896888158570401No Hit
GTTGGG72230.11868953476430112No Hit
TGGCGT72090.11845948444079285No Hit
GAATAC71960.11824586628324946No Hit
TCTGGG71540.11755571531272466No Hit
GGTGTT71350.117243504159392No Hit
GCTGGG70960.11660264968676183No Hit
GCTGCG70870.11645476019307795No Hit
GTGGCC70770.11629043853342919No Hit
GATCAC70660.11610968470781556No Hit
TGTAGG70660.11610968470781556No Hit
TTGGGA70140.115255212077642No Hit
CTTGGA69980.11499229742220397No Hit
TTCATG69540.11426928211974942No Hit
CCTATT69330.113924206634487No Hit
CTGGCG69320.11390777446852213No Hit
TTTGCC69160.1136448598130841No Hit
TTGTGG69100.11354626681729485No Hit
TACGTA68930.11326691999589196No Hit
GCGGTA68700.1128889801786998No Hit
TTGGTT68600.11272465851905103No Hit
GGCACG68230.11211666837835062No Hit
AAAGGA68200.11206737188045598No Hit
ATTAAC68130.11195234671870186No Hit
GCCGTC68080.11187018588887747No Hit
CTGCGG67870.11152511040361508No Hit
CGGGGT67720.11127862791414193No Hit
GGGTCG67700.11124576358221218No Hit
AAACGC67670.11119646708431755No Hit
TAGCAC67140.11032556228817911No Hit
TTCTCG67020.11012837629660059No Hit
GATATA66740.10966827564958406No Hit
GAGGGG66740.10966827564958406No Hit
ATGATT66730.10965184348361919No Hit
GCCTTG66030.10850159186607784No Hit
CGATAT65960.10838656670432371No Hit
AGCTAG65950.10837013453835884No Hit
CTGATG65840.10818938071274518No Hit
GGGCTC65750.10804149121906133No Hit
GTGTTG65630.1078443052274828No Hit
ATGGGG65590.1077785765636233No Hit
TGGCAC65410.10748279757625552No Hit
GCACTA65400.10746636541029064No Hit
GCCGGG65230.10718701858888775No Hit
TTGGCT64980.10677621443976584No Hit
TAATTC64940.10671048577590633No Hit
ATTTTG64870.1065954606141522No Hit
TTGTTT64600.10615179213310055No Hit
TCTATC64310.10567525932011913No Hit
TGCGAG64280.1056259628222245No Hit
TCGTAC64150.1054123446646811No Hit
GGGCAC63990.1051494300092431No Hit
GTGTGG63560.10444284687275343No Hit
ATTTTA63290.10399917839170175No Hit
TGTTAA63180.10381842456608811No Hit
CGTGGA63130.10373626373626373No Hit
GTGCAG63100.1036869672383691No Hit
GGGGTC63080.10365410290643934No Hit
GGGTTG62890.10334189175310671No Hit
GGATAG62740.10309540926363356No Hit
TTTGCT62630.10291465543801992No Hit
GTAGGC62530.10275033377837114No Hit
TGCTTT61870.10166581082468934No Hit
GGGCGG61820.10158364999486495No Hit
GTCCAT61690.10137003183732156No Hit
TCGAGT61490.10104138851802402No Hit
GGGGAT61380.1008606346924104No Hit
GGGGCT61330.10077847386258602No Hit
ATGGTG61080.1003676697134641No Hit
TCGACT61040.1003019410496046No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)