FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5051311
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT71328814.120849023154584Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT208650.4130610845382516Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT149940.29683383185078094Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT122900.24330317416607294Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110130.21802260838819865Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT54420.10773440795864676Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT481900.0131.98182
ACTGCAA489500.0130.20011
TGCAATT554400.0126.712393
CGCAATT69350.0122.204323
CCGCAAT66600.0117.5634462
ACCGCAA70950.0110.415691
AATTTCC804800.0106.778196
GCAATTT748800.0106.7414
CAATTTC786200.0106.169265
ATTTCCA829550.0103.283487
TTTCCAC827550.0102.998758
TTCCACA862400.098.870139
GACCGGA29600.083.763962
AGACCGG29900.083.365691
CGGCAAT17650.075.5780942
ACCGGAA37300.067.248993
GATTGGA52650.066.645342
GTTCCAC41650.066.1415255
AGATTGG53800.065.9176941
ACGGAAG33600.062.787364
Produced by FastQC (version 0.11.5)