FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10863947
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT8902178.194231801756764Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT262700.24180898526106578Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT196840.18118645092800986Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT179780.16548313426050404Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT157240.14473561036334215Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA929650.0126.8390051
CTGCAAT938550.0125.292342
TGCAATT1129150.0114.432743
CCGCAAT114050.0112.9596252
ACCGCAA117400.0109.6851961
CGCAATT125100.0109.586063
GCAATTT1438250.0100.2686544
AATTTCC1544300.099.523426
CAATTTC1500500.098.6116265
TTTCCAC1578400.096.7210858
ATTTCCA1592300.096.177257
TTCCACA1649350.092.881319
GACCGGA48100.077.774232
AGACCGG48850.077.740761
CGGCAAT29150.062.6748122
ACCGGAA64350.058.470923
GATTGGA90900.053.1977272
AGATTGG95150.051.304631
GTTCCAC83000.050.13665
GAACGGA57850.049.7528842
Produced by FastQC (version 0.11.5)