FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4466220
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4308359.64652435392793Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT169890.38038878514717145Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT157460.3525576438240839Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT119180.2668475802804161Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT96350.21573052827670827Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT378450.0130.840642
ACTGCAA387950.0127.093541
TGCAATT429100.0125.734813
CGCAATT50250.0119.780243
CCGCAAT47650.0118.240312
GCAATTT542550.0111.99554
ACCGCAA50350.0110.778411
AATTTCC606550.0110.510476
CAATTTC580000.0110.3221055
ATTTCCA627300.0106.331467
TTTCCAC625100.0106.205918
GACCGGA26450.0101.83922
TTCCACA662200.0100.408989
AGACCGG27750.096.8349461
CGGCAAT12100.082.20572
ACCGGAA34250.078.010673
GATTGGA47300.069.995552
AGATTGG47650.069.806521
GAACGGA33100.069.314782
AGAACGG32800.069.304891
Produced by FastQC (version 0.11.5)