FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7145831
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT146076320.442171106481528Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT433390.6064934924993328Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT328020.4590368845834725Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT295260.4131919716545213Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT261650.36615755396398264Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT114240.15986943995736816Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC97430.1363452340252659RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC95400.13350441677112151RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGACCTCCAGTCCATCTCGTATGCCGTCTT76770.10743327123185532Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC72780.10184959593922667Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT71550.10012831257834114Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA679400.0128.785921
CTGCAAT681450.0127.943132
TGCAATT819500.0117.40363
CCGCAAT84100.0112.842682
CGCAATT92450.0108.7667853
GCAATTT1055250.0101.713774
AATTTCC1123950.0101.676036
ACCGCAA93700.0101.4781651
CAATTTC1093850.0100.815355
GACCGGA58850.0100.4016652
AGACCGG59150.099.624671
TTTCCAC1142350.099.624428
ATTTCCA1151900.099.006847
TTCCACA1191700.095.7239159
ACCGGAA66500.088.414993
AGATTGG83950.088.3059841
GATTGGA84850.086.895822
ATTGGAA90850.081.635193
GAACGGA58700.077.8094252
ACGGAAG58950.077.355924
Produced by FastQC (version 0.11.5)