FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6799082
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT212772731.294327675412653Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA162640.23920876377134442No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC142410.20945474697907748RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC141420.2079986680554816RNA PCR Primer, Index 16 (96% over 25bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT141200.2076750949613492Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA137410.2021008130215226No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT115290.16956700919329992No Hit
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC107100.15752126537082506Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC105690.1554474559947946Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCAGTTTTTCTGTTCTGTTTTTTCTCCATCTTTGTGGTTTTATCTACTTT105220.15475618620278442No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT101430.14918190426295785No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA90900.1336945193483473No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG88530.1302087546524663No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA84600.12442856256182819No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT84230.12388437144896915No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG77610.1141477628891665No Hit
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC74940.11022076215583222RNA PCR Primer, Index 16 (100% over 22bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAC73230.10770571674234845No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAGAA73070.1074703908557067No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAGG70850.10420524417855234No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCAGG70520.10371988453735373No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAACA69720.10254325510414494No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAT69030.10152841221800236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1951200.0142.660631
CTGCAAT1969600.0140.62312
CCGCAAT279600.0136.788362
TGCAATT2439950.0131.382663
ACCGCAA294550.0130.099091
CGCAATT321850.0129.713673
CGGCAAT33550.0119.974142
ATTGCAA105600.0111.551071
GGCAATT41050.0101.948993
GCAATTT3765950.097.078144
CAATTTC3770850.096.892825
AATTTCC3765500.096.7165766
TTTCCAC3741200.096.598438
ATTTCCA3765000.096.399837
TTCCACA3771350.096.049149
ACGGCAA42600.094.563341
TATGCAA71100.091.225841
CATGCAA89600.090.163071
TTTGCAA67550.085.793751
AATGCAA132400.082.216021
Produced by FastQC (version 0.11.5)