FastQCFastQC Report
Thu 12 May 2022
EGAF00005479759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46350719
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC3824790.8251846104048568No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG988770.21332355167996425No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC867550.1871707750639208No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCA826750.1783683226143698No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA586230.1264770024387324No Hit
CAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGT540480.11660660539052263No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG514730.11105113601366141No Hit
CCAGTCTTTACAATCTAGTGCTGTTACTTTTCTTCTTGTGTGTACTCTGC494800.1067513105891626No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA473440.10214296783616238No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG469050.10119584121230138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)