FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25625207
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT3788071.4782592780616368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2959761.1550189623834064No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC2728731.0648616418981511No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT2551680.9957695171008765No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2539900.991172481065226No Hit
GAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA1516400.5917610733837194No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG1390690.5427039086942791No Hit
CGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGA1373490.5359917677933295No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGC1269060.495238926265064No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA1261380.4922418773046399No Hit
CCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCC1257870.4908721322719461No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA1215220.4742283642820915No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGG1203580.46968596195144885No Hit
CCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCG1147840.4479339425433715No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC1060780.41395958284356493No Hit
AGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAA846000.33014367454670707No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTT845680.3300187975066894No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA825250.3220461789830615No Hit
CCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCA789910.30825507087611037No Hit
CCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCT743610.29018692414855424No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC698690.2726573096560742No Hit
CACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCC640880.25009749189538255No Hit
AAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAA633000.24702239478494747No Hit
TGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGG629850.24579313642227357No Hit
GCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGC624820.24383022544949587No Hit
GGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAG596470.23276690018543073No Hit
CTCCGGAGCCCTCCTCCAGCCCTTCCTCCAGCCCTTCCTCCGGAGCCCTC582200.22719816468214285No Hit
TTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC559900.2184957959559117No Hit
GAAAAAGAACGGGAGAGAGAAAGGGAAGAAAGAGAAAGGAAAAGACGAAG531450.20739344661684098No Hit
CGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAG509090.19866766344560652No Hit
GGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACG507510.19805108306051927No Hit
CTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTCTT493310.1925096644097353No Hit
GGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGAT469420.18318681289091635No Hit
GCCCTTCCTCCGGAGCCCTCCTCCAGCCCTTCCTCCAGCCCTTCCTCCGG467870.18258193972833078No Hit
ATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA459430.1792883077978648No Hit
AGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGA458390.1788824574178074No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT449770.1755185821523315No Hit
CTTTCTTTTTCTCTTTCTTCCTCTTCCCTTCGTCTTTTCCTTTCTCTTTC449440.17538980270481327No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC435200.16983277442402708No Hit
GCCCTTTCTTTTTCTCTTTCTTCCTCTTCCCTTCGTCTTTTCCTTTCTCT427880.16697621213362296No Hit
GCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGC426700.16651572804855783No Hit
CTCCTCCAGCCCTTCCTCCAGCCCTTCCTCCGGAGCCCTCCTCCAGCCCT408590.15944846806505797No Hit
GAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATC407810.1591440802800149No Hit
CTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGC378470.14769441667339506No Hit
CCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACC361840.14120471299997694No Hit
GCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCAC358670.13996764982230192No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC355060.13855888071460262No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG350790.13689255271186687No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC336090.13115601368605528No Hit
CTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC331400.12932578456829635No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC330330.12890822696573728No Hit
CCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCT324410.12659800172541044No Hit
AAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGA323820.1263677596828779No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT323020.12605556708283372No Hit
GGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAG321040.12528289039772442No Hit
CTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC317260.1238077803625157No Hit
AAAGAACGGGAGAGAGAAAGGGAAGAAAGAGAAAGGAAAAGACGAAGGGA316210.12339802757495774No Hit
TCCAGCCCTTCCTCCGGAGCCCTCCTCCAGCCCTTCCTCCAGCCCTTCCT312690.12202438013476341No Hit
CTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTT309690.12085365788459777No Hit
GGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGG295270.11522638626880165No Hit
GCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACA294910.1150858995987818No Hit
GGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGG294150.11478931662873983No Hit
CCTTCCTCCAGCCCTTCCTCCGGAGCCCTCCTCCAGCCCTTCCTCCAGCC287070.11202641211834893No Hit
CAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGCCA278940.10885375482040009No Hit
CCTTCCTCCGGAGCCCTCCTCCAGCCCTTCCTCCAGCCCTTCCTCCGGAG277350.1082332720278123No Hit
GGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGA276640.1079562010952731No Hit
TCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCG273730.10682060051261245No Hit
GGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGG273720.10681669810511189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT1300.065.75025
TTAGCGA6000.055.4006276
GGTCGTA13650.053.2263535
CAGACCG505650.049.8387343
AGACCGC511350.049.4410554
ACCGCGT514500.049.1937336
CTATTGT29850.048.8384639
TCAGACC532050.048.0262262
CTCAGAC569500.045.8436241
GACGTTA6750.045.0240064
ACTAGAC11300.044.964866
ATACGCG750.044.32357834-35
GAGAAAT288700.043.768941
AATTGAA306500.043.6439745
GTTTACG7800.043.224676
CGACATA11450.043.131624
GAAATTG301200.042.7407843
TAGTGTA29350.041.4190182
CCGCGTT612150.041.3696337
TACGTTA3450.041.2923979
Produced by FastQC (version 0.11.5)