FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005479786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25625207
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6949272.7118883371361644No Hit
CGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTT4212191.6437681849750523No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC3220471.2567586283303No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT2460560.9602107799558458No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA2453420.9574244610004516No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG1941730.7577421716047016No Hit
CGCCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGT1797630.7015084795217459No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT1761910.6875690799297739No Hit
CCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTG1600790.6246934902808785No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA1572750.6137511396493305No Hit
CTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCCCG1305770.5095646641995907No Hit
CCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATC1142520.4458578617530778No Hit
GCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTC1080670.421721471362163No Hit
CTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGG1040290.40596354987493366No Hit
GAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA1016120.3965314309460993No Hit
TGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGG933600.36432876425154337No Hit
CCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTT890390.3474664614416578No Hit
CGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCC860710.3358841159800192No Hit
GGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAG771610.3011136651501001No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC763790.2980619824846683No Hit
GCCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTT632710.24690922496743145No Hit
ATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA551210.2151046038379319No Hit
TGAAGATGAAGAAGAGGATGATGAGGACGAGGATGACGACGACGACGAAG538860.2102851305747501No Hit
CCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCCC510950.1993935112407092No Hit
GCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGAT502400.19605695282773716No Hit
GGCTGGAGGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGGCTGGAGGAGGG485570.189489201004308No Hit
CGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGG484720.18915749636676105No Hit
CCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGTGGCGGT472370.18433802310357922No Hit
GGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGGCTGGAGGAGGGCTCCGGA470930.18377607642349972No Hit
AGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAA466270.18195755452824244No Hit
GAAAAAGAACGGGAGAGAGAAAGGGAAGAAAGAGAAAGGAAAAGACGAAG459990.17950684261789573No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA452720.17666979236499436No Hit
CGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATCG444730.17355176877205325No Hit
CTGGAGGAAGGGCTGGAGGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGGC443070.1729039691269616No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCA437000.17053520777412645No Hit
CGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACG429910.16776840085623504No Hit
GCTGGAGGAAGGGCTGGAGGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGG421830.16461525559578893No Hit
GGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCG395150.15420363238431598No Hit
CGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATT386620.15087487878634503No Hit
GTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGTGGCG381890.1490290400385839No Hit
AGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA379990.148287582613479No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTG376960.1471051531408117No Hit
GAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAA375810.1466563762782482No Hit
GGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACGAGAAAGGGAA362800.14157934412002995No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTG352150.13742328013194197No Hit
GCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGTG351820.13729450068442373No Hit
GGAAGGGCTGGAGGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGGCTGGAG350070.13661157937182714No Hit
CTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTCTT348840.1361315832492592No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTT348180.13587402435422277No Hit
GTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTT347280.1355228076791731No Hit
GCTGGAGGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGGCTGGAGGAGGGC339590.13252185631124852No Hit
TCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTT334600.13057455496847303No Hit
CTCCGGAGGAAGGGCTGGAGGAAGGGCTGGAGGAGGGCTCCGGAGGAAGG333480.1301374853284112No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTC330060.12880286196322238No Hit
CTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC324810.12675409802543253No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACT321450.12544288910524704No Hit
CCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCC316780.12362046480248921No Hit
AGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGA309740.12087316992210054No Hit
GGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGTGGCGGTGG303640.11849270134676375No Hit
GGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGT288090.11242445768340524No Hit
GGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAG282320.11017276855558669No Hit
CTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC278190.10856107425785869No Hit
TTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC272040.10616109364501915No Hit
GACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGG270100.10540402658991205No Hit
CGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTGTCTGTGGCGGTG268260.10468598360981043No Hit
AAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGA261570.1020752729919411No Hit
AAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGG257920.10065089425423958No Hit
TCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT256700.10017480053917223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)