FastQCFastQC Report
Thu 12 May 2022
EGAF00005479819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43987723
Sequences flagged as poor quality0
Sequence length25-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2278140.5179035977834088No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC1210310.27514722687509874No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG552180.1255304804024523No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG502550.11424778681997248No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG498400.11330434175917677No Hit
GCCAGACTCAGAAAAAAGGTGAAATTTAAAACGAACGAGAAGGGGAAGGG487310.11078318375333954No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTC453630.10312650191054444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)