FastQCFastQC Report
Sun 12 May 2024
EGAF00005479875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43296761
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1268640.29301037091435084No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG739390.1707725896632314No Hit
CTCGGTTTTATTCTTATTGCTCAGGCTTGAGTGCACTGGCCTCTTCTCAG519730.12003900245563404No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC484970.112010688282202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)