FastQCFastQC Report
Thu 12 May 2022
EGAF00005479881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34955049
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTA3298090.9435232089075315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3238360.9264355486956977No Hit
GCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACA2487550.7116425441142995No Hit
GCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCA2387410.6829943222222347No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACA2092140.5985229773243916No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1815500.5193813345820227No Hit
GTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGC1396830.3996075073446471No Hit
CTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCAC1385410.3963404542788654No Hit
CAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAGAA1287720.36839313256290956No Hit
ACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAG1262310.361123796450693No Hit
CTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTT1241820.3552619823247852No Hit
CCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACAC1175960.33642064126415616No Hit
GGCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACAC1069800.30605020751079476No Hit
CCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGT1066680.3051576325926478No Hit
AACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAGAAC1047460.29965914223149853No Hit
CCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAG724810.20735488026350646No Hit
AAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGA724000.20712315408283363No Hit
CACCACTCATCTTTGTGAGATGGGTCTCAGAGGGCCCCGTCCCTGCTGAC649570.18583009281434565No Hit
GTCTCAGAGGGCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCC594540.17008701661382308No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG581110.16624493932192744No Hit
ACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAGAACA539150.15424095099966817No Hit
GAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAA524560.15006701893051272No Hit
AGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAA479220.13709607444692753No Hit
CTCAGAGGGCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTG477470.13659543146399253No Hit
ACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATC416700.11921024627944306No Hit
CCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAGA415540.11887839150218327No Hit
TGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACA396060.1133055198978551No Hit
CGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAG395350.11310240188763575No Hit
AAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAG371670.1063279871242635No Hit
AAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAA366400.10482033654136773No Hit
GTGTGTGTCTCCCATTCTCTCTTCTCTCTCTGTCTCTCAGTCTCTGTGTG365880.1046715740550099No Hit
GACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCAT362790.10378758158799892No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC360340.10308668141188988No Hit
ACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGT349670.10003418962450888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)