FastQCFastQC Report
Thu 12 May 2022
EGAF00005479882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34955049
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8841242.52931700939684No Hit
GGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCT2133160.6102580488443887No Hit
AGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTC2009540.5748926285298585No Hit
GTTCTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAG1921480.5497002736285679No Hit
CCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTGTG1891740.5411922037357179No Hit
TGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTT1825630.5222793422489552No Hit
CTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGT1762170.5041245972792086No Hit
CTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTG1427550.4083959373079408No Hit
CCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGTAGCC1186160.339338674650406No Hit
GTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTG1185360.3391098092867786No Hit
GCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTGT1011750.2894431645625787No Hit
TAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTT992380.28390176194574923No Hit
CTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGTAGCCT944310.27014981440878544No Hit
GGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAAC886600.2536400392401109No Hit
CTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGT750970.21483877765412374No Hit
CTCAGTTCCTGTTCTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTG638970.18279762674628205No Hit
TTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTC589960.168776762407056No Hit
GTTCCTGTTCTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGT564280.16143018423461514No Hit
TGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTGTGTT530820.15185789040089745No Hit
CTTTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTT503440.14402497333074832No Hit
TCTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTG469220.13423525740158454No Hit
CCCATTCTCTCTTCTCTCTCCCTCTCTCAGTCTCTGTGTGCTTCTTTCCC436730.12494046282126511No Hit
CCTGTTCTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTG413380.11826045502038919No Hit
GCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACT390740.11178356522973261No Hit
AGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAA378150.10818179656964579No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCGCTG368870.10552695835156747No Hit
CTCTGTTCCTCCCCTTTCCGCCTAGGGGAAAGTCCCCGGACCTCGGGCAG368440.10540394321861771No Hit
AGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAA354260.10134730464832133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)