FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005479907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42985158
Sequences flagged as poor quality0
Sequence length25-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1175270.27341297663719183No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA965000.22449609234889864No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA679210.15801035324797458No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG589710.13718921307675547No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC461030.10725329891773341No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGC455930.10606684288563042No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG443140.10309139726786627No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCA437250.10172115687000616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)