FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005479933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45823372
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2539330.5541560756375589No Hit
GCCAGACTCAGAAAAAAGGTGAAATTTAAAACGAACGAGAAGGGGAAGGG1469150.32061149930214655No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG1392440.3038711337087982No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC1337560.2918947125933901No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA1294210.28243447470430594No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCA869850.18982671113771374No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA855490.18669293914031468No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG771910.16845333861506306No Hit
GGAAAAAGTAAAGAATAAGCCAGGGGGAATCAGAAACCACACAGCCCCAG622070.13575386813523893No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC527270.11506573545045964No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG513750.11211527602115356No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA491840.10733387320339498No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA474020.10344502800885103No Hit
GGCAGAGAAAGGAGAGGTAGACAGAACAAGAAAAGGACAAAAAGCACACA473830.10340356445178239No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT467390.10199816809640286No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTC463320.10110997505814283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)