FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005479934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45823372
Sequences flagged as poor quality0
Sequence length25-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA2251340.4913082345838713No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1892890.41308396073514625No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1531470.33421154602066383No Hit
CTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATA1005960.21952989404620854No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG904540.19739708374145842No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA854320.1864376109204709No Hit
CTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCC762600.1664216243186992No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA699810.15271900985374887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA656110.14318239172795927No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG639400.13953578099839534No Hit
CATGCTGTGTGCTTTTTGTCCTTTTCTTGTTCTGTCTACCTCTCCTTTCT634720.13851446811902013No Hit
CCTAGATGTGGCTCTTCTTTCACGCTTTATTTCATGTCTCCTTTTTGGGT580820.1267519116663872No Hit
CTCCTTTCTCTGCCTACCTCTCTTTTCTCTTTGTGAACTGTGATTATTTG570220.12443868164045196No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA527960.11521631363139316No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTT502900.1097474886832859No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA494120.1078314358882188No Hit
CTCTTCTTTCACGCTTTATTTCATGTCTCCTTTTTGGGTCACATGCTGTG480130.10477840871247973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)