FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16264166
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6676464.105012209049022Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT192320.11824768635539014Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1544000.0132.760162
ACTGCAA1572550.0130.917311
TGCAATT1718450.0128.849433
CGCAATT206200.0124.691713
CCGCAAT201900.0121.064732
ACCGCAA212700.0115.469611
GCAATTT2237400.0111.793024
AATTTCC2357150.0110.9191746
CAATTTC2321000.0109.887295
TTTCCAC2413850.0107.101288
ATTTCCA2438750.0106.7768557
TTCCACA2508600.0103.2525259
CGGCAAT48950.076.412322
ATGCAAT193000.056.262742
ACGGCAA65950.056.0983351
GGCAATT70800.055.594473
ATTGCAA113650.047.5828551
GTTCCAC125700.046.1915255
TCCGATC62100.045.4070245
GTAATTT132650.042.6783374
Produced by FastQC (version 0.11.5)