FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8677794
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5934066.838212568770357Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT176710.2036347025522846Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA107440.12381026790910224No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT98640.11366944179592187Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA87640.10099340915444639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1360350.0137.075131
CTGCAAT1357300.0136.516982
CCGCAAT175350.0131.499942
TGCAATT1518850.0130.55383
CGCAATT185600.0129.471223
ACCGCAA181350.0127.96811
GCAATTT1989250.0112.867944
AATTTCC2087950.0112.16
CAATTTC2041700.0111.747165
ATTTCCA2122400.0109.75797
TTTCCAC2110900.0109.5993968
TTCCACA2180500.0106.396929
CGGCAAT33650.097.5844962
ACGGCAA41500.079.16091
GTTCCAC73600.072.487435
GGCAATT52650.065.810813
CGTAATT9500.064.857923
AGACCGG31950.064.832731
GACCGGA32050.064.00682
ATTGCAA82200.063.0431751
Produced by FastQC (version 0.11.5)