FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101869
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT121534912.03093209781279Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT329500.326177264820995Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT179370.1775612017934503Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT162610.16097021254185734Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT151880.15034841572386257Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT128360.1270655954853503Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC125210.12394736063197812RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC117780.11659228604132563RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC103600.10255527962201845Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA101620.10059524628561309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1298950.0135.311312
ACTGCAA1311450.0134.405521
CCGCAAT179850.0129.459412
TGCAATT1476600.0128.619543
CGCAATT191150.0126.812043
ACCGCAA189250.0123.253971
GCAATTT1961600.0110.4208454
AATTTCC2060250.0109.728116
CAATTTC2020950.0109.3450555
TTTCCAC2085150.0107.22068
ATTTCCA2105050.0107.0008247
TTCCACA2167450.0103.460369
CGGCAAT36100.093.1706852
ACGGCAA47650.070.759841
AGACCGG44000.069.032781
GACCGGA45150.067.431072
GGCAATT53700.066.818923
GATTGGA87800.064.9753652
GTTCCAC84250.064.959935
AGATTGG90200.063.8849261
Produced by FastQC (version 0.11.5)