FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8966110
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1902472.121845482600593Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT674300.0126.720492
ACTGCAA681400.0126.050251
TGCAATT739850.0123.958243
CGCAATT94300.0118.761773
ACCGCAA91950.0116.673761
CCGCAAT91900.0116.026462
GCAATTT960450.0108.3398744
AATTTCC1037050.0105.480796
CAATTTC1012450.0105.138725
TTTCCAC1045750.0103.3243948
ATTTCCA1073650.0101.479377
TTCCACA1082100.0100.101369
CGGCAAT24550.062.5956652
ACGGCAA28600.053.11521
GTTCCAC60150.039.4070935
GGCAATT42500.038.204893
ATGCAAT98750.037.2894942
AGACCGG23400.036.341981
ATTGCAA69050.034.5261961
CGGAAGT35600.034.4111375
Produced by FastQC (version 0.11.5)