FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32938451
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA10965443.3290697246206267No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT2773180.8419278732931308No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA2027400.6155116401800437No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1772460.5381127363882412No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1760010.5343329593732261No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1161230.3525454187265819No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA1080160.3279328466296123No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG1035040.31423457041134084No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA933910.2835318515737124No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT847970.2574407642909498No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC789440.23967125837216813No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG780340.23690852979091215No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG771810.2343188512416689No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC757100.2298529460295507No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGC691440.20991879672787284No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC661770.20091108716678874No Hit
TTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCC651810.1978872655547767No Hit
CCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT618400.18774410490645113No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA603510.1832235523158026No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA556740.16902434179433634No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG533690.16202644137697914No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA523900.15905423117802353No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA520070.1578914564015169No Hit
AAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAG515720.15657081142036702No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG499520.15165254735263659No Hit
GGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTC494320.1500738453062046No Hit
CTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAA486950.14783633875193464No Hit
CTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG481690.14623942091265918No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC464050.14088397781668605No Hit
CAGTCTACACCCCTCAGCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACT455990.13843698964471643No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA450170.13667005773890217No Hit
CAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTAC440460.1337221352637378No Hit
GACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCAT431770.13108388126691203No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT427900.12990896262850976No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT425950.12931694936109778No Hit
GGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAG421170.12786575786456988No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG395230.11999046342525337No Hit
CTCAGACCGCATTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA357330.10848415427914325No Hit
TTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC351800.10680526537207229No Hit
TCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGAGCGTC348680.1058580441442131No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA343950.10442203247505476No Hit
GTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTG338220.10268242425850566No Hit
AAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAA331550.10065743528740925No Hit
GCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATA331450.10062707563267016No Hit
AGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGA330720.1004054501530749No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)