FastQCFastQC Report
Thu 12 May 2022
EGAF00005480002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32938451
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA2808370.8526114357958119No Hit
CCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATC2195410.6665188961071666No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT2137020.6487918937050197No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTG2004230.6084773081769996No Hit
GGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCT1790550.5436047979305402No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1744440.5296059611303519No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCA1400300.4251262453112929No Hit
CACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTG1203440.3653602289919462No Hit
CGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATT1159590.35204752038886106No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG1085030.32941136181540537No Hit
GCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGAT1063670.32292653956313855No Hit
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGC934540.2837231173985686No Hit
CGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATCG924280.28060821682233933No Hit
GCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAA889190.26995501397439725No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT788150.23927961882603405No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA756070.22954024158573821No Hit
GTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTG745310.22627354273581354No Hit
CTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCT711240.21593000836621004No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA699990.2125145472080639No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT676330.20533145289679833No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT670120.20344611833750165No Hit
TTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATG658510.19992136242229486No Hit
TGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGG651440.19777493483224212No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA621810.18877936913305365No Hit
CGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTT599320.18195148278223527No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACT598110.18158413095989243No Hit
CTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGGTG591930.1797079042970175No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA591790.1796654007803828No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA585060.1776221960164429No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC583000.17699678712881792No Hit
GAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTT563560.1710948702475414No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG541260.16432466724072725No Hit
CTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCCCG525790.15962802865259207No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA524880.15935175579446648No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA510480.1549799655120394No Hit
TGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGC499850.15175273421327554No Hit
GAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCC496800.1508267647437337No Hit
GGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAG479760.145653479576195No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT476350.1446182153495925No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA470600.14287253520209556No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT455770.13837019840429046No Hit
CGCTCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTGCGGGAAGGGA452050.13724081924799683No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT450720.1368370358399671No Hit
CTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAG449080.13633913750224624No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCG444290.13488491004024444No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG433450.1315939234665285No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG414840.12594399171958634No Hit
GAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGG413890.1256555749995651No Hit
CTTCCCTTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC409660.12437136160410214No Hit
CTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGATGTGGGAG392300.11910092554139841No Hit
ATCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC386350.11729452608442335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376480.11429802816167645No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC351130.10660185568532048No Hit
GCCCTACGGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTT350110.1062921872069819No Hit
CCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTG346940.10532978615175315No Hit
TATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGT341020.1035324945911998No Hit
TGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCC340970.10351731476383028No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT336170.10206005133635458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)