FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51677085
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT8456221.6363577783073486No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1093130.21153089420581672No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC812290.15718572361424799No Hit
CTGTTTCCAAATTGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCT607240.11750662793770197No Hit
TCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG532980.10313662235398921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)