FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005480043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34402210
Sequences flagged as poor quality0
Sequence length25-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT28359418.243484938903634No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG3408340.9907328628015468No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT3067130.8915502812173984No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA3015740.8766122874082799No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC2630370.764593321184889No Hit
TCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG2219230.6450835571319401No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC900330.2617070240545593No Hit
TCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG895170.2602071204146478No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA891640.25918102354470834No Hit
TCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA890440.2588322087447289No Hit
GCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT850250.24714981973541816No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT804870.23395880671619645No Hit
TGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGG776790.22579654039667799No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG652740.18973781044880547No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA651080.18925528330883395No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA614380.17858736400946335No Hit
TTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTC607510.17659039927958115No Hit
GACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCAT555290.16141114190047673No Hit
AGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA550870.16012634072055254No Hit
TCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT550090.1598996111005659No Hit
TGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG483300.14048516069171138No Hit
TTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAA473720.13770045587187568No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT473440.1376190657518805No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC430980.12527683541260867No Hit
GTTCTCGTTCCCGCTCCCGTTCTTTCTCCTTTTCTCGTTCACGTTCTCTT424000.12324789599272837No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC422720.12287582687275032No Hit
TTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCT406960.11829472583302061No Hit
GCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATCT403400.11725990859308166No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG402950.11712910304308938No Hit
GGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAG388600.11295785939333547No Hit
CCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCG378880.11013245951350219No Hit
TCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTC378730.11008885766350476No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG367970.10696115162368929No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG362630.10540892576378087No Hit
TTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCA358250.104135751743856No Hit
GGCAGTTGCAGCCAAGCAATGCCAGAAAGCCGGCTTCACGCTCAGGAGAA353220.10267363637394225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)