FastQCFastQC Report
Thu 12 May 2022
EGAF00005480044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34402210
Sequences flagged as poor quality0
Sequence length25-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTG3612491.0500749806480456No Hit
CACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTG3154470.9169381850759005No Hit
GGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCT2947050.856645546899458No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT2375450.6904934305092609No Hit
CCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATC2055830.5975866085347424No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT2045490.5945809876749197No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1903360.5532667814073572No Hit
CTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCT1857650.5399798443181412No Hit
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGC1690460.4913812223110085No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG1630430.473931761942038No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT1495420.4346871901543535No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCA1423860.4138862009155807No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCATTGCTTGGCTG1309320.3805918282575451No Hit
GCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGAT1278110.3715197366680803No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA1269280.36895304109823174No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC1220870.354881270709062No Hit
CGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATT1168350.33961480962996277No Hit
GGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCATTGCTTGGCT1150090.3343070110902759No Hit
AGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG987550.2870600464330635No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACT984770.28625195881311116No Hit
CTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGG896300.2605355876846284No Hit
CCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCT883920.2569369816648407No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA794740.23101422844637015No Hit
CGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATCG710370.2064896412178171No Hit
CGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTT707740.20572515544786224No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG706470.20535599311788402No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA686440.1995336927482275No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCATTGCTTGGCTGCA659300.19164466468869296No Hit
GCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGC626240.18203481694925994No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG616860.17930824792942082No Hit
CGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACG616240.17912802694943145No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA608700.17693630728956078No Hit
AGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT584160.16980304462998164No Hit
TGAAGATGAAGAAGAGGATGATGAGGACGAGGATGACGACGACGACGAAG568280.16518706211025397No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC567560.1649777732302663No Hit
CTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAA558250.16227155174042596No Hit
CCGGCTTTCTGGCATTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATC556180.1616698462104615No Hit
CGTGAAGCCGGCTTTCTGGCATTGCTTGGCTGCAACTGCCGTCAGCCATT549960.15986182283056816No Hit
TAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGC543790.15806833340067397No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCG540270.15704514332073433No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG525270.1526849583209916No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC515840.14994385535115332No Hit
GAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTT486880.14152579151164998No Hit
CTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCCCG448880.1304799895123017No Hit
CTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAG447330.13002943706232828No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA425310.1236286854827059No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA420730.12229737566278447No Hit
AAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGC411890.11972777330293606No Hit
GTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTG408230.11866388816299883No Hit
GCCGGCTTTCTGGCATTGCTTGGCTGCAACTGCCGTCAGCCATTGATGAT407840.11855052335300552No Hit
CGCCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGT396310.11519899448320327No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA394410.11464670438323585No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCATTGCTTGGCTGCAACT392450.11407697354326946No Hit
GAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGG390680.11356247171329982No Hit
GAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTG390530.1135188698633024No Hit
CTCTGTTCCTCCCCTTTCCGCCTAGGGGAAAGTCCCCGGACCTCGGGCAG389860.11332411493331389No Hit
CACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGC377930.10985631446351848No Hit
CGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATCGTTCTTCTCTCC372540.10828955465361091No Hit
CGACGATGAAGTCAGTGAGGAGGAAGAAGAATTTGGACTTGATGAAGAAG370540.10770819665364521No Hit
CCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTG370510.10769947628364573No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCA365490.10624026770373182No Hit
CGAAGAAGAGGAAGAAGGTGGGGAGGAAGAGGAGGAGGAAGAAGAAGGTG363260.10559205353377007No Hit
GGAAGAAGGTGGGGAGGAAGAGGAGGAGGAAGAAGAAGGTGATGGTGAGG349250.10151964074401035No Hit
TGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGG346610.10075224818405562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)