FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48515335
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2467810.5086659712851617No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1698130.35001922587981715No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1245430.25670852319168774No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG1243360.2562818539746247No Hit
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA1208830.2491645167450663No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT1137690.23450111186493922No Hit
GCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGG1011370.20846398360435933No Hit
CGCTGGTGGACTGGTAGGTGGAAGAATCCTCAGGGTTGAAGCGGTTGTGG861590.17759127088373192No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA834090.17192296002903No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG827080.1704780560620678No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC788310.16248676835891165No Hit
GTGCTTCTTCAGGAAGTCCTTCAGCAGGCCACGCTCGGACAGGGTGCGCC728020.1500597697614579No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT719990.14840462299188492No Hit
CTCGCTGGTGGACTGGTAGGTGGAAGAATCCTCAGGGTTGAAGCGGTTGT716210.1476254878998568No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA701570.14460788532120822No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTT666890.13745963003244233No Hit
CTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGAC666080.137292672512722No Hit
GCTGGGTTGAGGTTGTGCTTCTTCAGGAAGTCCTTCAGCAGGCCACGCTC658940.1358209728944467No Hit
CCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGTGGA655840.13518199967082573No Hit
GGAGACTGTCTCGCTGGTGGACTGGTAGGTGGAAGAATCCTCAGGGTTGA645490.13304865358551066No Hit
GTGGACTGGTAGGTGGAAGAATCCTCAGGGTTGAAGCGGTTGTGGTTGGT601000.12387835722457652No Hit
CGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGTGGAA579230.11939111623159976No Hit
CCGGTGTCAAAGACGACGGTGAAATCCTGGGCAGGAGTTCCGATGCCGAT569370.11735876913969573No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC545360.11240981846255417No Hit
CTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCA543230.11197078202180816No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG521930.10758041761434811No Hit
CTGGTGGACTGGTAGGTGGAAGAATCCTCAGGGTTGAAGCGGTTGTGGTT521630.1075185814959332No Hit
CCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAG512100.10555425413428558No Hit
GTGGAAGAATCCTCAGGGTTGAAGCGGTTGTGGTTGGTGCAGGCAAGACT508770.10486787321987985No Hit
GGACTGGTAGGTGGAAGAATCCTCAGGGTTGAAGCGGTTGTGGTTGGTGC507800.10466793643700491No Hit
CTCAGGGTTGAAGCGGTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGA486700.10031879610848818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)