FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48515335
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC1702810.3509838693270901No Hit
CCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGG1027820.21185466409744466No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA792960.1634452281943431No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG787710.1623630961220818No Hit
CAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACCAGTCCACCA757740.1561856678924303No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC725520.14954446877466682No Hit
CGGAACTCCTGCCCAGGATTTCACCGTCGTCTTTGACACCGGCTCCTCCA724320.14929712430100708No Hit
CCAGAGTGGCAGCGTGGTGATCTTTGGTGGCATTGACTCTTCTTACTACA688740.14196336065699638No Hit
CCGGCTCCTCCAACCTGTGGGTGCCCTCAGTCTACTGCTCCAGTCTTGCC685110.14121514362417573No Hit
CTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCG665640.13720197953904678No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC652430.1344791291248427No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA645180.13298475626314857No Hit
CTTTGGTGGCATTGACTCTTCTTACTACACTGGAAGTCTGAACTGGGTGC635150.13091736870414272No Hit
CTCCCACCCTGGTAGATGAACAGCCCCTGGAGAACTACCTGGATATGGAG615300.12682587886902152No Hit
GGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGGATTTCACCG569580.1174020544225862No Hit
TGAACAGCCCCTGGAGAACTACCTGGATATGGAGTACTTCGGCACTATCG563630.1161756380740234No Hit
CTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCACC554590.11431230970578685No Hit
CTGGAAGTCTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATC535580.11039396100222744No Hit
CTTCTCTGTCTACCTCAGCGCCGATGACCAGAGTGGCAGCGTGGTGATCT508830.10488024044356284No Hit
CTTTGACACCGGCTCCTCCAACCTGTGGGTGCCCTCAGTCTACTGCTCCA505210.10413408461468936No Hit
CTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAACC493950.10181316897018232No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG491280.10126282751628944No Hit
CGGAACTCCTGCCCAGGATTTCACTGTCCTCTTTGACACCGGCTCCTCCA488140.10061560947687984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)