FastQCFastQC Report
Thu 12 May 2022
EGAF00005480150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33257116
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATG1583020.4759943706483749No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA688370.20698427368145814No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA656250.1973261902806004No Hit
TCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATC629010.18913546201661022No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC521800.1568987521347311No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTT445530.13396531437061468No Hit
ATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCAT410300.12337209275753196No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT404090.12150482320836238No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTG367880.11061692781779393No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG352410.10596529175891259No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG350390.1053579029522584No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC341270.10261563269647314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)