FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35414768
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2249770.6352632325588015No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT1942600.5485282298051479No Hit
GCCGGCTTCACGCTCAGGAGAAAAACGCTACCTCTCTTCCTCGTGGTTTT1441710.40709288283351175No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1191290.3363822685496627No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG868280.24517455542840205No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG833910.23546956456131524No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG754960.21317660474297048No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA749150.21153604620535704No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG734200.20731464342784908No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCA679590.1918945226465976No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA662670.1871168547539264No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTC650120.1835731353654498No Hit
CTCAGGAGAAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACA617100.1742493414046931No Hit
CTCATATCTCATAATCTTCGCTCACAGGACGCTGGACATTCAAAGAAAGA561740.1586174445643693No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC556030.15700512283463214No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTC549610.1551923197689732No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGT548840.15497489634832565No Hit
GCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG407750.11513558411564351No Hit
CCGGAGTTTACGAGCTAGTCTCTGTCATTGAGCCATCAAGAAGGAAAAAC378490.10687349413103595No Hit
TGACGGATGACGCCCCCGCGCCACGCCGCTCAGCGGGATACGCTTCTTGG371540.10491103598363259No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC366340.10344272197406461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)