FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005480305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27945075
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC3045361.089766264717486No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC548660.19633513239810593No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG540440.19339364807573428No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT443110.15856461290585194No Hit
GCCGGCTTCACGCTGAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC418140.1496292280482339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGC991100.039.1808787
GCCGGCT935800.038.6897541
TCACGCT1006800.038.414228
CACGCTC893150.037.7665259
CCGGCTT1029300.037.3947452
CGGCTTC1061400.036.7604453
GACCGCG89850.035.0994875
CTTCACG1111750.034.9459576
AGACCGC94200.033.6300284
ACCGCGT93900.033.3832866
GCTTCAC1201100.033.29145
GGCTTCA1213600.032.9016574
ATTACGC41800.031.360547
TTACGCA44250.029.8388638
CAGACCG113900.028.480613
GATTACG54050.025.1316576
TACGCAG52900.024.8699469
ACTATAG688850.024.71285288-89
CCGCGTT128850.024.475667
CTACACG930400.024.45607656-57
Produced by FastQC (version 0.11.5)