FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005480306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27945075
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1105450.3955795430858568No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC713280.25524354470331534No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA438890.1570545078157779No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA399220.14285880427946607No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA354290.1267808370526828No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG334670.11975992191826287No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA318460.1139592575793767No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT306810.10979036556530981No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA305330.10926075524935969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCA103400.033.88293594-95
GCGTACG22850.029.26196394-95
GCGTCGG37500.027.42319594-95
TTTCTAT630900.020.1335531
TCTATAG711800.020.0173633
TTCTATA726200.019.8136562
CGTACGG16450.019.7290394-95
AGCGTCG52650.019.68072394-95
GTTCTAT99100.019.4744031
AGGGATC207850.019.0909142
GCGACCC111700.018.8513075
CTATAGT763150.018.6349724
CGACCCG112800.018.547726
TATAGTG783900.018.2970375
ATAGTGT785850.018.2345836
GGCGACC114600.018.1381634
TAGTGTG818150.017.515017
TGGCGAC119500.017.3944533
CGTGGCG124150.016.7435781
TAACGCG13500.016.6998754
Produced by FastQC (version 0.11.5)