FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005480313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38742821
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1295150.3342941909160409No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC990240.25559315879450284No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG937240.24191320502964922No Hit
TCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG478820.12358934833372097No Hit
TTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC454300.11726043387496228No Hit
TGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG450680.11632606722158925No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA415480.10724051302304496No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)