FastQCFastQC Report
Thu 12 May 2022
EGAF00005480319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36428000
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA31375758.613086087624904No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC8316012.2828620841111236No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG5715751.569054024376853No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA4911281.348215658284836No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA2788990.7656171077193368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2583270.7091440650049413No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA2315220.6355605578126716No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1782880.4894257164818272No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG1368110.37556549906665204No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG1263450.3468348523114088No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC1236070.3393186559789173No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1021120.2803118480289887No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT970820.2665037882947184No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG715730.19647798396837599No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA701890.19267870868562645No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA691420.18980454595366203No Hit
ACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATC664020.18228285933897004No Hit
GACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCAT655490.1799412539804546No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA636830.17481882068738333No Hit
CGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGA617790.16959207203250248No Hit
CTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAG592910.16276216097507412No Hit
CCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCC582660.15994839134731526No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGC548770.15064510815855936No Hit
CAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTAC532300.14612386076644338No Hit
CTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTCTT513070.14084495443065775No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT496320.13624684308773471No Hit
GCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA492150.1351021192489294No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGC485690.13332875809816624No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA485360.13323816844185793No Hit
CACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCC457760.12566157900516087No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC439230.12057483254639288No Hit
TCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA438380.12034149555287142No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGC435340.11950697265839465No Hit
CTCGGTTTTATTCTTATTGCTCAGGCTTGAGTGCACTGGCCTCTTCTCAG423960.11638300208630724No Hit
CTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG420560.11544965411222137No Hit
CCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTC410770.1127621609750741No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA405880.11141978697705061No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC399720.10972878005929504No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG386240.10602832985615461No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG385600.10585264082573845No Hit
GAAAAAGAACGGGAGAGAGAAAGGGAAGAAAGAGAAAGGAAAAGACGAAG380310.10440046118370483No Hit
CTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC379760.10424947842319096No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)