FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005480327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43973113
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA15282413.475398705568105No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC3343930.7604487769605941No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG3192150.7259322304518218No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA2153900.4898220419373084No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1498030.34066953595029764No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA709160.16127127501753175No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC641480.14588005174889482No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT521550.11860656760871126No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA510330.1160550084320844No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT470830.10707224662488644No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG465870.10594428463593197No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA456910.10390667588169161No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)