FastQCFastQC Report
Thu 12 May 2022
EGAF00005480328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43973113
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG2103790.47842644208518964No Hit
CACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTG1929030.43868397490985No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1751980.39842073496138425No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTG1520730.34583178134329495No Hit
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGC1441620.3278412424428537No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA1389810.31605904271548846No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT1199770.2728417248967568No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1162200.26429786765380925No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT1097740.24963891003122748No Hit
CCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATC1074900.24444482700144518No Hit
CTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCT1018920.23171432052126942No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCA926240.21063780496959586No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC923770.21007609809203182No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC896800.20394280477709187No Hit
GGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCT877980.19966291674642184No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA851570.1936569739786219No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCG851490.19363878104331617No Hit
GCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGAT743100.16898962782098234No Hit
CGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATT678090.15420559376817375No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA569750.129567811130406No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG549220.12489904910757627No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACT508820.11571161677818899No Hit
TAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGC502960.11437898426704518No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA498640.11339656576053644No Hit
GAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGG466130.10600341167567554No Hit
AAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGC463660.10544170479811152No Hit
GAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTT451650.10271049038534069No Hit
CCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCT440140.10009298181823059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)