FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40174636
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC3289890.8188972763810479No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1525210.37964500786018324No Hit
GCCAGACTCAGAAAAAAGGTGAAATTTAAAACGAACGAGAAGGGGAAGGG860810.21426703156688215No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG791500.19701485285392506No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG786210.19569810165797147No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC708570.17637247540960918No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCA687610.17115525327970613No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT646500.16092242876824076No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG528260.13149092377588686No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC524610.13058239034200583No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA427510.10641291186807517No Hit
GCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG403950.10054851523732536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)