FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005480389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54054008
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1159130.21443923270222628No Hit
CTCAGACCGCGTTTTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1042080.19278496425278954No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA1009540.18676505912383037No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC954650.1766104004720612No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC713140.13193101240522256No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA601710.111316444841611No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG592550.10962184339781057No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC591160.10936469317871858No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACTTCTCTTCCTCGTGGTTTTC555460.10276018755167979No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)