FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43365581
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT10676562.4619893827780146No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT1116090.25736770366341916No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1025060.23637640182890665No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC933950.21536665218436715No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG646190.14900987951712213No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA585830.13509100685172418No Hit
TTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCC507910.11712283988539207No Hit
TTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAA480310.11075834542606498No Hit
TCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG459900.10605184789291766No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG446580.10298028752341631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)