FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45242957
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT11912102.6329180915385346No Hit
TTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC923160.20404501854288612No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC916240.2025154987106612No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG840150.18569741142251156No Hit
GATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGG757850.16750673480515432No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG709130.1567382078938828No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC677800.14981337316214766No Hit
ATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTC507970.11227603889816486No Hit
TCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG490020.10830857054723456No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG472190.10436762566160297No Hit
TTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC468600.1035741319914169No Hit
TGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTT455760.1007361212044562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)