FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005480595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28365764
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1241950.4378341440054285No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1085140.3825527138983459No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC386100.13611478964571516No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG380950.13429922070845685No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC372290.13124624459260112No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG341140.12026469655462128No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA328340.1157522145358045No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG326230.11500836007801517No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG308210.1086556314858997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)