FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005480616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29731752
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA834590.2807066330971683No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC424460.14276319807860635No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT380740.12805838014524001No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG334820.11261361254459543No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT300170.1009594052849627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG36550.036.3288494-95
CGCGTAA3900.026.4780621
CGTACGG21800.025.44070294-95
TCTATAG417100.023.9062983
GGCGTAC49950.022.53068494-95
TTTCTAT446150.022.4513071
GCGTCGG49750.021.80754594-95
CTATAGT453350.021.7767684
CGTTTCG6450.021.5791919
ATAGTGT461050.021.5689496
TATAGTG460550.021.4948465
TTCTATA468750.021.3209652
TAGTGTG505550.019.5375067
AGCGTCG55850.018.99079594-95
CGTGGCG171350.018.0271591
TGGCGAC170700.017.9635093
GTGGCGA173450.017.498282
GCGACCC174800.017.4393735
GGCGACC178500.017.153314
CGCGGTC51350.017.09532788-89
Produced by FastQC (version 0.11.5)