FastQCFastQC Report
Thu 12 May 2022
EGAF00005480617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31938559
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4417321.38306803384586No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2629350.8232525456142213No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1915870.5998611271097108No Hit
TGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1728090.5410669905301614No Hit
TCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG1451280.45439745731797104No Hit
TCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG1354990.4242489462345499No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG1347260.4218286742366805No Hit
TTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1336470.41845031267691196No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1145130.35854153595345367No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG1108890.3471947497693932No Hit
GCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACA861590.2697648319074132No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA789560.24721215506310099No Hit
GACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGT766650.24003900739541817No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTC752260.23553348164518004No Hit
TCATATCTCATAATCTTCGCTCACAGGACGCTGGACATTCAAAGAAAGAA750360.23493858943354332No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC741850.23227409852773886No Hit
TCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG740940.23198917646848125No Hit
GCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCG728320.22803783977855732No Hit
CCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAG663120.2076236438844971No Hit
TCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAA620460.19426674822743253No Hit
TCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA612850.19188404836924547No Hit
TGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG576790.1805936203947085No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT561080.17567480110796482No Hit
GCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT557070.17441926544024733No Hit
GGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT530690.16615965673341745No Hit
GGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGT512620.1605019186995882No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA497020.15561754054088664No Hit
GGGTAGTACAGTCAGCCCTGTGATACTCTTCAGTGAAGGCTGCTCTCTCC469970.14714815405416382No Hit
AGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT462980.1449595769176687No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC437130.13686591182776905No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA407520.12759498636115674No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC396130.1240287641029766No Hit
GCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACT371690.1163765716543442No Hit
TCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCTA361190.11308900943214126No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA358040.11210274076548037No Hit
GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC356660.11167066115913368No Hit
CCCAATTCAATCTGGGCCGCCACAAAGACAGACAGAAAGCGCAGGGATTT347950.10894355002052535No Hit
CAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTG341730.10699606078032513No Hit
CAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAA338870.10610059145122984No Hit
AGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGG338490.10598161300890249No Hit
AGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACC332350.10405917186182383No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC329820.10326702591685491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)