FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48009583
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2760790.5750497770413878No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2357050.4909540663996186No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG890380.18545880725520986No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG787750.16408182508063027No Hit
TCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG647210.13480850271080255No Hit
TGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT575040.1197760872032569No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA501080.10437082946544235No Hit
TGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG497050.10353141371796544No Hit
GATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGG487630.10156930544470673No Hit
TCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG486350.10130269200630217No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)