FastQCFastQC Report
Thu 12 May 2022
EGAF00005480685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40871666
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA6977031.7070578918901913No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA5484081.3417803913351611No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC3134260.7668539863288176No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA2550840.6241096215652183No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2534930.6202169493164287No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA2363100.5781755996929511No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA2276660.5570264740370505No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC1913860.46826082401436725No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA1106310.27067895886602716No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT1097910.268623745359438No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1080500.26436407069875745No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1076480.2633805042348898No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGC873840.21380092507117276No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC796270.19482200701092048No Hit
TCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG766520.18754312584175062No Hit
CTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAG732320.1791754708506377No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG699500.17114545807846443No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG684610.1675023474697606No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG657710.16092077088318346No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC641160.1568715109386537No Hit
TCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA589500.14423194787313048No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG568850.13917954800276552No Hit
CCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGG489130.11967459315213626No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG459080.1123223115005882No Hit
GCCGGCTTCACGCTCAGGACAAAACGCTACCTCTCTTCCTCGTGGTTTTC454080.11109897012761849No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC446860.10933246518505021No Hit
GCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCA445230.10893365589746207No Hit
CTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG441150.10793540933711877No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG434990.10642825276562008No Hit
CCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTG434970.10642335940012819No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT414250.10135383275054168No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA412700.10097459692492104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)