FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005480686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40871666
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG4047310.9902483544468189No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG3293370.8057831554994602No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT2815470.6888561870710139No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA2570220.628851292726849No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1840200.4502385589077773No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA1502780.36768258969428846No Hit
CCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATC1309250.3203319385121223No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG1263080.3090356042741199No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTGTCCTGAGCGTGAAGCCG1249920.30581576978046354No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1243060.3041373454167491No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT1242490.3039978845002306No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA1158320.2834041558276582No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC1150730.28154712362349016No Hit
TTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT1055650.2582840640750979No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCA1035910.2534543123346134No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA1033230.25279860135870164No Hit
CTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCT909490.2225233490604469No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT883790.2162353744033825No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT867000.2121273940729502No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACT849150.20776006537144828No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTG807960.19768217914092368No Hit
GAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCC764970.18716389001613No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA751800.18394160883972777No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA742300.18161726023108526No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC730360.17869592103243356No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG694750.16998328377414318No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG652950.15975614989611628No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA632110.1546572630535785No Hit
AGCGTTTTGTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCA603480.14765241035195387No Hit
GTAGCGTTTTGTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTG600250.14686213182501542No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA560620.13716592810285735No Hit
TCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC558920.13674999203604765No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT557880.13649553703046996No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTGTCCTGAGCGTGAAGCCGG536710.13131590965731615No Hit
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGC529380.12952249120454254No Hit
AAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGC516250.12630999675912405No Hit
CACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTG511010.12502793500025175No Hit
GTTTTGTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACT510500.12490315418020885No Hit
GCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAA504560.12344982462912082No Hit
CGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTCAGCCATTGATGATCG499710.1222631834973402No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA476440.11656975274753908No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCG473890.11594584864732453No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC472780.11567426686252524No Hit
GAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTG457940.1120433896675511No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTGTC442790.10833666530745284No Hit
AGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG434120.10621539136672334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)