FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005480690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005480690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38804732
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1768170.4556583459975964No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1021540.26325139933964753No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA988730.2547962449528063No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC987060.2543658850678314No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA892760.2300647250958981No Hit
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC792060.2041142817324444No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG781030.20127184488737096No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC585890.1509841634777944No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG582250.15004613354886717No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT580850.1496853528069721No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA572370.1475000523132076No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG560110.14434064381632633No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA527210.1358622963817918No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG524600.13518969799868738No Hit
CCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGG495760.1277576147156486No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC466090.12011163999277202No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA435130.1121332315862921No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT431120.1110998524612926No Hit
CCGGCTCCTCCAACCTGTGGGTGCCCTCAGTCTACTGCTCCAGTCTTGCC417930.10770078247158105No Hit
CGGAACTCCTGCCCAGGATTTCACTGTCCTCTTTGACACCGGCTCCTCCA399010.10282508844539887No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG393790.10147989167919005No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT393720.1014618526420953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)