FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005481210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66207
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15422.3290588608455303No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14052.122132100835259No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12151.8351533825728399No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11561.7460389384808255No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9511.4364040056187413No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9441.425831105472231No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9371.4152582053257208No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8321.256664703128068No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8161.232498074221759No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8131.2279668313018262No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7841.184164816409141No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7771.173591916262631No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7631.1524461159696104No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7461.126769072756657No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7401.1177065869167913No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7111.0739045720241063No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6731.0165088283716224No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6330.9560922561058498No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6230.9409881130394068No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA6060.9153110698264534No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6040.9122902412131647No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5940.8971860981467217No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5840.8820819550802785No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5450.8231757971211503No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5330.8050508254414186No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5240.7914570966816198No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5240.7914570966816198No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5080.7672904677753107No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4950.7476550817889347No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4940.7461446674822905No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4780.7219780385759814No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4730.7144259670427598No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4430.6691135378434304No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4430.6691135378434304No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4360.6585406376969203No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4270.6449469089371215No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3950.5966136511245035No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3930.5935928225112148No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3890.5875511652846376No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA3860.5830199223647047No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3790.5724470222181944No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3750.5664053649916172No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3700.5588532934583956No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3630.5482803933118854No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3440.5195825214856435No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3380.5105200356457776No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA3370.5090096213391333No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3220.48635340673946864No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3040.45916594921987103No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3040.45916594921987103No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2990.4516138776866494No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2960.4470826347667165No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA2950.4455722204600722No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2870.4334889060069177No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA2860.43197849170027336No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2810.4244264201670518No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2810.4244264201670518No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2790.4214055915537632No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2730.4123431057138974No Hit
GGACTACTCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2700.4078118627939644No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2650.4002597912607428No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2530.3821348195810111No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA2510.3791139909677224No Hit
GGACTACCCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2390.3609890192879907No Hit
GGACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2350.3549473620614134No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2220.3353119760750374No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA2210.33380156176839304No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA2180.32927031884846014No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2160.32624949023517147No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA2070.3126557614753727No Hit
GGACTACACGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1980.2990620327155739No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1970.2975516184089296No Hit
GGACTACTGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1930.29150996118235234No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1910.28848913256906367No Hit
GGACTACCGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1910.28848913256906367No Hit
GGACTACTCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1900.2869787182624194No Hit
GGACTACCCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1830.2764058181159092No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1820.2748954038092649No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1800.27187457519597624No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1800.27187457519597624No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1790.27036416088933196No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1780.2688537465826876No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1740.2628120893561104No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1710.25828084643617744No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1700.2567704321295331No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1610.24317670336973432No Hit
GGACTACAGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1600.24166628906309No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1600.24166628906309No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1590.24015587475644568No Hit
GGACTACCGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1580.2386454604498014No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA1580.2386454604498014No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1560.23562463183651275No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1530.23109338891657982No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1530.23109338891657982No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.22052048877006963No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1440.21749966015678102No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1410.2129684172368481No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1350.2039059313969822No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1340.20239551709033787No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1320.19937468847704923No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1320.19937468847704923No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1300.19635385986376064No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1300.19635385986376064No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1290.19484344555711633No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1260.1903122026371834No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.1903122026371834No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1250.18880178833053907No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1240.18729137402389476No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1240.18729137402389476No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1220.18427054541060614No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1220.18427054541060614No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1200.1812497167973175No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1190.1797393024906732No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1150.17369764526409592No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1130.1706768166508073No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1130.1706768166508073No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.1676559880375187No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1110.1676559880375187No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.16463515942423007No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.16312474511758573No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1080.16312474511758573No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1070.16161433081094145No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1050.1585935021976528No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1050.1585935021976528No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.1585935021976528No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1050.1585935021976528No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1030.1555726735843642No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1000.15104143066443124No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1000.15104143066443124No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA980.14802060205114262No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA970.1465101877444983No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC960.144999773437854No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA930.14046853051792105No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA910.13744770190463246No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA910.13744770190463246No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA900.13593728759798812No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA900.13593728759798812No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA890.1344268732913438No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.1298956303714109No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA850.12838521606476655No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC840.12687480175812227No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.12536438745147793No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA820.12385397314483362No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA810.1223435588381893No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA800.120833144531545No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.120833144531545No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA800.120833144531545No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC790.1193227302249007No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA790.1193227302249007No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG770.11630190161161208No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.11479148730496776No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.11479148730496776No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.11177065869167913No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.11177065869167913No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA730.11026024438503482No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA730.11026024438503482No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.10874983007839051No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA710.10723941577174619No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA700.10572900146510186No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA690.10421858715845757No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT680.10270817285181326No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)